The K m for ATP in soluble fraction was 12.8 Λ 1.5 and in the mitochondrial fraction was 5.3 ± 1.6. The K m for glycerol in the soluble fraction was 6.3 ± 0.1 μM and in the mitochondrial fraction was 4.0 = 0.3 μM. Steadystate kinetic parameters of glycerol kinase activity were compared in the soluble and mitochondrial fractions.
No major differences in distribution were noted between these tissues of the total homogenate activity, 0–20% sedimented with the nuclear fraction, 24–36% sedimented with the post-nuclear fraction and 62–69% remained soluble. Whole adrenal, adrenal cortex and adrenal medulla were examined for distribution of glycerol kinase between soluble and particulate fractions.
The subcellular distribution and substrate kinetics of soluble and particulate-associated bovine adrenal glycerol kinase have been investigated.
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